Journal: PLoS ONE

Article Title: Functional Insights into Recombinant TROSPA Protein from Ixodes ricinus

doi: 10.1371/journal.pone.0076848

Figure Lengend Snippet: I. ricinus TROSPA protein-coated ELISA microplates were incubated with the serial dilutions of rabbit TROSPA antiserum or rabbit preimmune antiserum. After washing, the plates were treated with B. garinii OspA protein (C = 30 µg/ml, 100 µl/well), which was subsequently detected by FITC-conjugated anti -Borrelia goat polyclonal IgG. The X axis represents serial sera dilutions, and the Y axis represents the level of fluorescence counts. The level of TROSPA-OspA binding was determined for ten rabbit TROSPA antiserum or preimmune serum concentrations. The level of OspA binding was determined as the arithmetic mean of fluorescence counts based on 16 reactions performed for every TROSPA antiserum or preimmune serum dilution (for details see Materials and methods). The reduction of fluorescence counts was statistically significant (p<0,01) for all serum dilutions except the highest one.

Article Snippet: TROSPA-coated ELISA microplates (prepared as described above) were incubated for 90 min at room temperature with 100 µl/well serially diluted (in PBST) rabbit TROSPA antiserum or rabbit preimmune serum (Eurogentec).

Techniques: Enzyme-linked Immunosorbent Assay, Incubation, Fluorescence, Binding Assay